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Promega
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Thermo Fisher
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Image Search Results
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: HyperXpress: Rapid Single Vessel DNA Assembly and Protein Production in Microliterscale
doi: 10.3389/fbioe.2022.832176
Figure Lengend Snippet: HyperXpress workflow and effect on product formation of the most relevant optimizations. (A) The HyperXpress workflow starts with the assembly of DNA parts using LCR. This assembly includes a circularization of the DNA construct which upon addition of the components required for RCA results in an amplification of circular constructs. In order to provide optimal reaction conditions for CFPS a buffer exchange is achieved through DNA precipitation and resuspension. Upon addition of the components required for CFPS each DNA assembly is assayed for product formation. (B) Results of the sequential optimizations of the workflow compared to the corresponding 0 nM bridging oligo reaction as a negative control (Neg.). EP29: EquiPhi29™ DNA Polymerase; N2(sN)2N2: endonuclease protected random hexamer oligonucleotides; PEG prec.: in-well DNA precipitation using polyethylenglycol; res.: resuspension of the DNA precipitate in ultrapure water; p09005 coexp.: bicistronic operon plasmid with sfGFP and mKate2 genes used instead of p10024 template with monocistronic GFP reporter construct ( n = 6).
Article Snippet: Moreover, two phi-polymerases were tested to achieve a higher RCA efficiency: The wild type polymerase with a temperature optimum at 30°C (
Techniques: Construct, Amplification, Buffer Exchange, Negative Control, Random Hexamer Labeling, Plasmid Preparation
Journal: Journal of Personalized Medicine
Article Title: Field of Genes: An Investigation of Sports-Related Genetic Testing
doi: 10.3390/jpm2030119
Figure Lengend Snippet: Companies providing sports-related DNA tests or analyses.
Article Snippet: CyGene Direct , “Our athletic & sports analysis will help you learn more about your athletic gene as well as help you improve athletic performance” and
Techniques: